MICA antibody (AA 68-97)

Catalog No. ABIN652566

Product Details anti-MICA Antibody

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Binding Specificity: AA 68-97

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.

Clone: RB21777

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. IF: 1:25. WB: 1:2000. WB: 1:2000. WB: 1:2000. WB: 1:2000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MICA antibody (ABIN652566)

Shi, Li, Couturier, Yang, Guo, He, Lewis, Zhou: "Allele Specific Expression of MICA Variants in Human Fibroblasts Suggests a Pathogenic Mechanism." in: The open rheumatology journal, Vol. 9, pp. 60-4, (2015) (PubMed).

Andresen, Skovbakke, Persson, Hagemann-Jensen, Hansen, Jensen, Skov: "2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 4, pp. 1847-55, (2012) (PubMed).

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA (MICA Products)

Synonyms: MIC-A antibody, PERB11.1 antibody, MHC class I polypeptide-related sequence A antibody, MICA antibody

Background: MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Molecular Weight: 42915

Gene ID: 4276

NCBI Accession: NP_000238

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response