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Retinoblastoma 1 antibody (C-Term)

Name: Retinoblastoma 1 antibody (C-Term)
SKU: ABIN652516
Price: 451 USD
Availability: InStock

RB1 Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal RB20715 unconjugated

All lanes : Anti-RB1 Antibody (C-term) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. (Retinoblastoma 1 antibody (C-Term))

RB1 Antibody (C-term) (ABIN652516 and ABIN2842345) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram). (Retinoblastoma 1 antibody (C-Term))

FACS

Western blot analysis of RB1 Antibody (C-term) (ABIN652516 and ABIN2842345) in 293 cell line lysates (35 μg/lane). (Retinoblastoma 1 antibody (C-Term))

Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Retinoblastoma 1 antibody (C-Term))

IHC (p)

4 images

Catalog No. ABIN652516

Datasheet as PDF

Target See all Retinoblastoma 1 (RB1) Antibodies

Retinoblastoma 1 (RB1)
Binding Specificity
28

20

20

19

18

17

15

13

6

6

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 858-886, C-Term
Reactivity
230

106

91

5

4

3

3

3

2

2

1

1

1
Human
Host
204

29
Rabbit
Compatible Secondaries
Clonality
191

42
Polyclonal
Conjugate
127

8

7

6

6

6

6

6

6

6

6

6

6

6

6

2

2

2

2

2

2

1

1

1

1

1

1

1
This Retinoblastoma 1 antibody is un-conjugated
Application
186

80

79

66

63

39

30

29

20

16

10

8

8

6

6

2

2
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity

Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1.

Clone

RB20715

Isotype

Ig Fraction

Top Product

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anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 Reactivity: Human, Rat WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 Reactivity: Human WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807) antibody
RB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (AA 586-615) antibody
RB1 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB7977 unconjugated

anti-Retinoblastoma 1 (RB1) (pSer612) antibody
RB1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB7659 unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 Reactivity: Human WB, IF, IP, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated

Application Notes

WB: 1:8000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Target

Retinoblastoma 1 (RB1)

Alternative Name

RB1 (RB1 Products)

Background

RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.

Molecular Weight

106159

Gene ID

5925

NCBI Accession

NP_000312

UniProt

P06400

Pathways

Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy