IL-1 beta antibody (AA 148-174)

Catalog No. ABIN652470

Product Details anti-IL-1 beta Antibody

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Binding Specificity: AA 148-174

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Pr, Rb

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 amino acids of human IL1B.

Clone: RB21917

Isotype: IgG

Application Details

Application Notes: WB: 1:2000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-IL-1 beta antibody (ABIN652470)

Abdul-Muneer, Alikunju, Mishra, Schuetz, Szlachetka, Burnham, Haorah: "Activation of NLRP3 inflammasome by cholesterol crystals in alcohol consumption induces atherosclerotic lesions." in: Brain, behavior, and immunity, Vol. 62, pp. 291-305, (2018) (PubMed).

Liu, Wen, Chen, Wang, Huang, Tu: "Effects of Berberine on NLRP3 and IL-1β Expressions in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: BioMed research international, Vol. 2016, pp. 2503703, (2016) (PubMed).

Zhang, Liu, Huang, Su, Zhang, Wang, Li: "NF-?B activation and cell death after intracerebral hemorrhage in patients." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 35, Issue 7, pp. 1097-102, (2014) (PubMed).

Liu, Tu, Chen, Wang, Hu, Dong: "Effects of Modified Simiao Decoction on IL-1 β and TNF α Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 406816, (2014) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B (IL1B Products)

Synonyms: IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody

Background: IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (,CNS), is found to contribute to inflammatory pain hypersensitivity.

Molecular Weight: 30748

Gene ID: 3553

NCBI Accession: NP_000567

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome