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ENOS antibody (N-Term)

Name: ENOS antibody (N-Term)
SKU: ABIN652267
Price: 429 USD
Availability: InStock

NOS3 Reactivity: Human IF, FACS, IHC (p) Host: Rabbit Polyclonal RB18211 unconjugated

Confocal immunofluorescent analysis of NOS3 Antibody (N-term) (ABIN652267 and ABIN2841142) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (ENOS antibody (N-Term))

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ENOS antibody (N-Term))

IHC (p)

NOS3 Antibody (N-term) (ABIN652267 and ABIN2841142) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). (ENOS antibody (N-Term))

FACS

3 images

(1 reference)

Catalog No. ABIN652267

Datasheet as PDF

Target See all ENOS (NOS3) Antibodies

ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
Binding Specificity
33

25

25

22

17

16

16

15

11

10

9

8

8

8

5

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 48-75, N-Term
Reactivity
267

151

147

13

10

7

7

6

3

2

2

1

1

1
Human
Host
277

24

1
Rabbit
Compatible Secondaries
Clonality
279

23
Polyclonal
Conjugate
114

25

17

14

9

8

8

8

8

8

8

8

8

8

8

8

5

5

5

5

5

5

5
This ENOS antibody is un-conjugated
Application
250

104

98

91

76

51

36

25

25

25

11

8

7

4

3

1
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

B, Pig

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This NOS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human NOS3.

Clone

RB18211

Isotype

Ig Fraction

Top Product

Discover our top product NOS3 Primary Antibody
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody
NOS3 Reactivity: Human, Rat, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1105-1202) antibody
NOS3 Reactivity: Human, Rat, Mouse WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody
NOS3 Reactivity: Human, Rat, Mouse WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (C-Term) antibody
NOS3 Reactivity: Human, Rat, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1156-1183) antibody
NOS3 Reactivity: Human WB, IF, FACS Host: Rabbit Polyclonal RB29786 unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1-120) antibody
NOS3 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) antibody
NOS3 Reactivity: Human WB Host: Rabbit Monoclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1156-1183) antibody
NOS3 Reactivity: Human WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 756-888) antibody
NOS3 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pThr495) antibody
NOS3 Reactivity: Human, Mouse WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Dabrowska, Uram, Zielinski, Rode, Sikora: "Oxidative stress and inhibition of nitric oxide generation underlie methotrexate-induced senescence in human colon cancer cells." in: Mechanisms of ageing and development, Vol. 170, pp. 22-29, (2018) (PubMed).
Target

ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name

NOS3 (NOS3 Products)

Synonyms

ECNOS antibody, eNOS antibody, 2310065A03Rik antibody, Nos-3 antibody, ecNOS antibody, EC-NOS antibody, NOSIII antibody, cNOS antibody, NOS antibody, NOS3 antibody, eNos antibody, ENOS antibody, nitric oxide synthase 3 antibody, nitric oxide synthase 3, endothelial cell antibody, NOS3 antibody, Nos3 antibody, nos3 antibody

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.

Molecular Weight

133275

Gene ID

4846

NCBI Accession

NP_000594, NP_001153581, NP_001153582, NP_001153583

UniProt

P29474

Pathways

ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling