TPTEa antibody (N-Term)
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
RB4973
unconjugated
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- Target See all TPTEa products
- TPTEa
- Binding Specificity
- AA 42-74, N-Term
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This TPTEa antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- Immunogen
- This TPTEa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-74 amino acids from the N-terminal region of human TPTEa.
- Clone
- RB4973
- Isotype
- Ig Fraction
-
-
- Application Notes
- WB: 1:1000. IHC-P: 1:10~50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
- Expiry Date
- 6 months
-
- Target
- TPTEa
- Abstract
- TPTEa Products
- Background
- Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
- Gene ID
- 7179
- NCBI Accession
- NP_954868, NP_954869, NP_954870
- UniProt
- P56180
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