Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
B, C, Rat
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human BTG1.
BTG1
Reactivity: Human
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Zhang, Yang, Cai, Zhao, Sun, Li, Feng, Feng, Ye, Niu, Zhang: "miR-511 promotes the proliferation of human hepatoma cells by targeting the 3'UTR of B cell translocation gene 1 (BTG1) mRNA." in: Acta pharmacologica Sinica, Vol. 38, Issue 8, pp. 1161-1170, (2018) (PubMed).
BTG1 is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8,12)(q24,q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia.