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Hsc70 antibody (N-Term)

Name: Hsc70 antibody (N-Term)
SKU: ABIN650754
Price: 451 USD
Availability: InStock

HSPA8 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB17655 unconjugated

Western blot analysis of lysates from A431,Hela,mouse NIH/3T3, rat H-4-II-E cell line (from left to right),using HS Antibody (N-term) (ABIN650754 and ABIN2839488). (Hsc70 antibody (N-Term))

Formalin-fixed and paraffin-embedded human brain tissue reacted with HS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Hsc70 antibody (N-Term))

IHC (p)

Confocal immunofluorescent analysis of HS Antibody (N-term) (ABIN650754 and ABIN2839488) with cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Hsc70 antibody (N-Term))

HS Antibody (N-term) (ABIN650754 and ABIN2839488) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). (Hsc70 antibody (N-Term))

FACS

4 images

(2 references)

Catalog No. ABIN650754

Datasheet as PDF

Target See all Hsc70 (HSPA8) Antibodies

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Binding Specificity
14

6

6

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 82-110, N-Term
Reactivity
83

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human
Host
71

28

7

2
Rabbit
Compatible Secondaries
Clonality
68

39
Polyclonal
Conjugate
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
Application
94

51

41

30

29

28

15

13

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity

B, C, Ha, Hs, M, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8.

Clone

RB17655

Isotype

Ig Fraction

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HSPA8 Reactivity: Human WB, IHC, ELISA, IP, FACS Host: Mouse Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibody
HSPA8 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16528 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA Host: Mouse Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio), Goat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Fitter, Gronthos, Ooi, Zannettino: "The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70." in: Stem cells (Dayton, Ohio), Vol. 35, Issue 4, pp. 940-951, (2017) (PubMed).

Li, Li, Wang, Cui, Gong, Qu, Zhong, Zhou, Zhou, Gao, Li: "Quantitative proteomic study of human prostate cancer cells with different metastatic potentials." in: International journal of oncology, Vol. 48, Issue 4, pp. 1437-46, (2016) (PubMed).
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name

HSPA8 (HSPA8 Products)

Background

HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

Molecular Weight

70898

Gene ID

3312

NCBI Accession

NP_006588, NP_694881

UniProt

P11142