PTRH2 antibody (AA 80-110)

Catalog No. ABIN650622

Product Details anti-PTRH2 Antibody

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Binding Specificity: AA 80-110

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTRH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Bit1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the Central region of human Bit1.

Clone: RB06095

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PTRH2

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Alternative Name: Bit1 (PTRH2 Products)

Synonyms: BIT1 antibody, PTH2 antibody, A230072I16Rik antibody, Bit1 antibody, CGI-147 antibody, wu:fj09f02 antibody, zgc:109954 antibody, RGD1306819 antibody, peptidyl-tRNA hydrolase 2 antibody, ptrh2 antibody, CC1G_10259 antibody, ANI_1_1036184 antibody, AOR_1_2082154 antibody, PTRH2 antibody, Ptrh2 antibody

Background: Adhesion to extracellular matrix regulates cell survival via integrin engagement and cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. A role for Bit1 (Bcl-2 inhibitor of transcription 1) has been identified in this process. Bit1 is a mitochondrial protein released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. Bit1-AES complexes may switch off a survival-promoting gene transcription program controlled by TLE. Apoptosis of Bit1/AES transfected cells is inhibited when cells are permitted to attach to fibronectin through the alpha-beta integrin, suggesting that the contribution of the Bit1-AES pathway to anoikis is regulated by integrins.

Molecular Weight: 19194

Gene ID: 51651

NCBI Accession: NP_057161

UniProt: Q9Y3E5