Western Blotting (WB), ELISA, Functional Studies (Func)
Purpose
Rabbit Anti-Mouse MCP-1 / CCL2
Specificity
DS-PB-01286 recognizes mouse monocyte chemotactic protein-1 (MCP-1), also known as CCL2 and JE. The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts. MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.
Characteristics
Cytokines/Chemokines/Growth Factors/Receptors
Purification
Purified
Immunogen
Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
ELISA (dilution: 0.1, 0.2 μg/mL), Functional Assays, Western Blotting
Restrictions
For Research Use only
Reconstitution
Reconstitute with 0.1 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09 % sodium azide is recommended. N.B. For functional studies do not add sodium azide