ASCL1 antibody (AA 79-91)

Catalog No. ABIN6391392

Product Details anti-ASCL1 Antibody

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Binding Specificity: AA 79-91

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Purpose: ASCL1 (aa79-91)

Sequence: HKSAPKQVKR QRS

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Mouse and Rat Lung lysates (calculated MW of 24.7 kDa according to Mouse NP_032579.2 and 24.9 kDa according to Rat NP_071779) . Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1 (ASCL1 Products)

Synonyms: ASH1 antibody, HASH1 antibody, MASH1 antibody, bHLHa46 antibody, AI225900 antibody, Mash1 antibody, ASCL1 antibody, Zash-1a antibody, ash antibody, asha antibody, id:ibd5085 antibody, im:7138587 antibody, si:dkey-264k15.2 antibody, zash1a antibody, zasha antibody, Xash1 antibody, ascl1-A antibody, Zash-1b antibody, ashb antibody, zashb antibody, achaete-scute family bHLH transcription factor 1 antibody, achaete-scute family bHLH transcription factor 1a antibody, achaete-scute family bHLH transcription factor 1 L homeolog antibody, achaete-scute complex homolog 1 antibody, achaete-scute family bHLH transcription factor 1b antibody, ASCL1 antibody, Ascl1 antibody, ascl1a antibody, ascl1.L antibody, LOC100220505 antibody, ascl1b antibody

Background: ASCL1, achaete-scute complex homolog 1 (Drosophila), ASH1, HASH1, MASH1, bHLHa46, ASH-1, achaete scute protein, achaete-scute complex-like 1, achaete-scute homolog 1, class A basic helix-loop-helix protein 46

Gene ID: 429, 17172, 64186

NCBI Accession: NP_004307

Pathways: Dopaminergic Neurogenesis