Nodal antibody (Internal Region)

Catalog No. ABIN6391350

Product Details anti-Nodal Antibody

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Nodal antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: NODAL

Sequence: DRSQLCRKVK FQ

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in Rat Brain lysates (calculated MW of 40.5 kDa according to NP_001099864.1 ). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this p

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Nodal

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Alternative Name: NODAL (NODAL Products)

Background: NODAL, nodal growth differentiation factor, HTX5

Gene ID: 4838

NCBI Accession: NP_060525

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity