PPP1R12A antibody (C-Term)

Catalog No. ABIN6391349

Product Details anti-PPP1R12A Antibody

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: PPP1R12A

Sequence: REDEYKQKYS RTYD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 4-5 μg/mL. Preliminary testing showed positive staining of the neuronal cells and neuropils in paraffin embedded Human Cortex.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A (PPP1R12A Products)

Synonyms: 1200015F06Rik antibody, 5730577I22Rik antibody, AA792106 antibody, AV099298 antibody, D10Ertd625e antibody, Mypt1 antibody, M130 antibody, MBS antibody, MYPT1 antibody, M110 antibody, MBSP antibody, protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody, protein phosphatase 1 regulatory subunit 12A antibody, protein phosphatase 1, regulatory subunit 12A antibody, Ppp1r12a antibody, PPP1R12A antibody

Background: Protein phosphatase 1, regulatory (inhibitor) subunit 12A, MBS, MGC133042, MYPT1, myosin phosphatase, target subunit 1

NCBI Accession: NP_002471, NP_001231919, NP_001231921, NP_001137358

Pathways: M Phase