PPP1R12A antibody (C-Term)
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- Target See all PPP1R12A Antibodies
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PPP1R12A antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- PPP1R12A
- Sequence
- REDEYKQKYS RTYD
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 4-5 μg/mL. Preliminary testing showed positive staining of the neuronal cells and neuropils in paraffin embedded Human Cortex.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- Alternative Name
- PPP1R12A (PPP1R12A Products)
- Background
- Protein phosphatase 1, regulatory (inhibitor) subunit 12A, MBS, MGC133042, MYPT1, myosin phosphatase, target subunit 1
- NCBI Accession
- NP_002471, NP_001231919, NP_001231921, NP_001137358
- Pathways
- M Phase
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