HDAC2 antibody (Internal Region)

Catalog No. ABIN6391348

Product Details anti-HDAC2 Antibody

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HDAC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Hdac2 (mouse)

Sequence: PEDAVHEDSG DE

Specificity: This antibody imay cross-react with HDAC1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3 kDa according to NP_032255.2). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells and additionally in the membrane and cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Alternative Name: Hdac2 (HDAC2 Products)

Synonyms: HD2 antibody, RPD3 antibody, YAF1 antibody, D10Wsu179e antibody, Yy1bp antibody, mRPD3 antibody, HISTONE DEACETYLASE antibody, T1N24.9 antibody, T1N24_9 antibody, histone deacetylase 2 antibody, histone deacetylase 2 antibody, histone deacetylase 2 S homeolog antibody, HDAC2 antibody, Hdac2 antibody, hdac2.S antibody, HDA2 antibody

Background: Hdac2, histone deacetylase 2, D10Wsu179e, YAF1, Yy1bp, mRPD3, HD2, OTTMUSP00000022803, YY1 transcription factor-binding protein

Gene ID: 3066, 15182, 84577

NCBI Accession: NP_032255

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection