LAMP1 antibody (PE)

Catalog No. ABIN638446

Product Details anti-LAMP1 Antibody (PE)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Human, Mouse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human PBMC

Clone: H4A3

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-LAMP1 antibody (PE) (ABIN638446)

Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).

Mao, Tu, Liu, Qin, Zheng, Chan, Lam, Peiris, Lau: "Inhibition of human natural killer cell activity by influenza virions and hemagglutinin." in: Journal of virology, Vol. 84, Issue 9, pp. 4148-57, (2010) (PubMed).

Yu, Gallegos, Marches, Zurawski, Ramilo, García-Sastre, Banchereau, Palucka: "Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines." in: Blood, Vol. 112, Issue 9, pp. 3671-8, (2008) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, (2007) (PubMed).

Marcenaro, Gallo, Martini, Santoro, Griffiths, Aricó, Moretta, Pende: "Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease." in: Blood, Vol. 108, Issue 7, pp. 2316-23, (2006) (PubMed).

Deetz, Hebbeler, Propp, Cairo, Tikhonov, Pauza: "Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys." in: Infection and immunity, Vol. 74, Issue 8, pp. 4505-11, (2006) (PubMed).

Furuta, Ikeda, Nakayama, Nakamura, Tanaka, Hamasaki, Himeno, Hamilton, August: "Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases." in: The American journal of pathology, Vol. 159, Issue 2, pp. 449-55, (2001) (PubMed).

Mane, Marzella, Bainton, Holt, Cha, Hildreth, August: "Purification and characterization of human lysosomal membrane glycoproteins." in: Archives of biochemistry and biophysics, Vol. 268, Issue 1, pp. 360-78, (1989) (PubMed).

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: CD107a (LAMP1 Products)

Synonyms: CD107a antibody, LAMPA antibody, LGP120 antibody, AI196048 antibody, Lamp-1 antibody, LEP100 antibody, LAMP-1 antibody, LGP-A antibody, Lgpa antibody, Lysosome-associated membrane protein 1 antibody, cd107a antibody, lamp-1 antibody, lamp1 antibody, lampa antibody, lgp120 antibody, rexp76 antibody, lysosomal associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 L homeolog antibody, LAMP1 antibody, Lamp1 antibody, lamp1.L antibody

Background: Lysosomal associated membrane protein 1,CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2 % of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.,LAMP-1, LAMPA

Gene ID: 3916

UniProt: P11279

Pathways: Autophagy