NAP1L4
Reactivity: Human
ELISA, WB, Neut
Host: Rabbit
Polyclonal
APC
Application Notes
Optimal conditions to be detrmined by user. Indirect: To detect hNAP-2 byIndirect ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech's Biotinylated Anti-Human NAP-2 (500-P03GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. To detect hNAP-2 by WB analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
Lot specific
Buffer
Lyophilized from PBS, pH 7.2.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
Target
NAP1L4
(Nucleosome Assembly Protein 1-Like 4 (NAP1L4))
NAP-2 is a CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is produced in leukocytes by enzymatic processing of a precursor called platelet basic protein (PBP). NAP-2 chemoattracts and activates neutrophils.