NAP1L4 antibody

Catalog No. ABIN636541

Product Details anti-NAP1L4 Antibody

Target: NAP1L4 (Nucleosome Assembly Protein 1-Like 4 (NAP1L4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NAP1L4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Blocking Antibody (Inhibition)

Purification: Purified

Immunogen: NAP2 antibody was raised in goat using highly pure recombinant hNAP-2 as the immunogen.

Application Details

Application Notes: Optimal conditions to be detrmined by user. Indirect: To detect hNAP-2 byIndirect ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech's Biotinylated Anti-Human NAP-2 (500-P03GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. To detect hNAP-2 by WB analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for NAP1L4

Target: NAP1L4 (Nucleosome Assembly Protein 1-Like 4 (NAP1L4))

Alternative Name: NAP2 (NAP1L4 Products)

Background: NAP-2 is a CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is produced in leukocytes by enzymatic processing of a precursor called platelet basic protein (PBP). NAP-2 chemoattracts and activates neutrophils.

Molecular Weight: 7.6 kDa (predicted detection band MW)