MCM10 antibody (Middle Region)
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- Target See all MCM10 Antibodies
- MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))
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Binding Specificity
- Middle Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MCM10 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- MCM10 antibody was raised against the middle region of MCM10
- Purification
- Affinity purified
- Immunogen
- MCM10 antibody was raised using the middle region of MCM10 corresponding to a region with amino acids PKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERV
- Top Product
- Discover our top product MCM10 Primary Antibody
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- Application Notes
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WB: 1 µg/mL
Optimal conditions should be determined by the investigator. - Comment
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MCM10 Blocking Peptide, catalog no. 33R-7173, is also available for use as a blocking control in assays to test for specificity of this MCM10 antibody
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of MCM10 antibody in PBS
- Concentration
- Lot specific
- Buffer
- PBS
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- Target
- MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))
- Alternative Name
- MCM10 (MCM10 Products)
- Synonyms
- CG9241 antibody, DmMCM10 antibody, Dmel\\CG9241 antibody, Scim19 antibody, mcm10 antibody, CNA43 antibody, DNA43 antibody, xmcm10 antibody, 2410041F14Rik antibody, AU018508 antibody, C330019M07Rik antibody, C79164 antibody, Minichromosome maintenance 10 antibody, minichromosome maintenance 10 replication initiation factor antibody, minichromosome maintenance 10 replication initiation factor L homeolog antibody, Mcm10 antibody, MCM10 antibody, mcm10.L antibody
- Background
- This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.
- Molecular Weight
- 98 kDa (MW of target protein)
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication
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