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beta Actin antibody (Middle Region)

ACTB Reactivity: Human, Mouse, Rat, Dog, Drosophila melanogaster, C. elegans WB Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN631092

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
39

16

14

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region
Reactivity
175

125

107

55

43

41

37

25

23

22

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human, Mouse, Rat, Dog, Drosophila melanogaster, C. elegans
Host
114

76

5

1

1
Rabbit
Compatible Secondaries
Clonality
111

84
Polyclonal
Conjugate
123

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
164

68

51

46

34

31

26

22

13

13

8

6

6

5

5

2

1

1
Western Blotting (WB)

Specificity

Beta Actin antibody was raised against the middle region of ACTB

Purification

Affinity purified

Immunogen

beta Actin antibody was raised using the middle region of ACTB corresponding to a region with amino acids TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK

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anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow WB, ELISA, IHC (p), FACS, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human WB, IF, IP Host: Mouse Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 359-368) antibody
ACTB Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

Application Notes

WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment

Beta Actin Blocking Peptide, catalog no. 33R-9203, is also available for use as a blocking control in assays to test for specificity of this Beta Actin antibody

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Lyophilized powder. Add distilled water for a 1 mg/mL concentration of ACTB antibody in PBS

Concentration

Lot specific

Buffer

PBS

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

beta Actin (ACTB Products)

Background

This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Molecular Weight

42 kDa (MW of target protein)

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction