DDOST antibody (N-Term)

Catalog No. ABIN630468

Product Details anti-DDOST Antibody

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDOST antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: DDOST antibody was raised against the N terminal of DDOST

Purification: Purified

Immunogen: DDOST antibody was raised using the N terminal of DDOST corresponding to a region with amino acids SPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFD

Application Details

Application Notes: WB: 5 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

DDOST Blocking Peptide, catalog no. 33R-8698, is also available for use as a blocking control in assays to test for specificity of this DDOST antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of DDOST antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for DDOST

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: DDOST (DDOST Products)

Background: DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Molecular Weight: 46 kDa (MW of target protein)

Pathways: Cell RedoxHomeostasis