Mitochondrial Marker antibody
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- Target
- Mitochondrial Marker
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- Purpose
- Mouse anti-Human Mitochondria Marker Antibody [Sodium Azide Free]
- Specificity
- Mitochondria in cytoplasm
- Purification
- The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
- Immunogen
- The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
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- Application Notes
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FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Buffer
- In 1X PBS ( pH 7.4), BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Target
- Mitochondrial Marker
- Alternative Name
- Mitochondria Marker
- Background
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Target Description: MAb MTC754 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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