Nuclear Antigen antibody

Catalog No. ABIN6297660

Product Details anti-Nuclear Antigen Antibody

Target: Nuclear Antigen (IPO-38)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Antigen antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: Mouse anti-Human/Mouse/Rat IPO-38 Antibody (Proliferation Marker) [Sodium Azide Free]

Specificity: Nuclear

Purification: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.

Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Nuclear Antigen

Target: Nuclear Antigen (IPO-38)

Alternative Name: IPO38 (IPO-38 Products)

Background:

Target Description: Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.