Nuclear Marker antibody

Catalog No. ABIN6296760

Product Details anti-Nuclear Marker Antibody

Target: Nuclear Marker

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Marker antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purpose: Mouse anti-Human/Mouse/Rat Nuclear Marker Antibody [Sodium Azide Free]

Specificity: Nuclei

Purification: Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.

Immunogen: Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Nuclear Marker

Target: Nuclear Marker

Abstract: Nuclear Marker Products

Background:

Target Description: This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.