PRKAA1/PRKAA2 antibody (Thr172, Thr183)
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- Target See all PRKAA1/PRKAA2 products
- PRKAA1/PRKAA2 (AMPK1/AMPK2)
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Binding Specificity
- AA 110-190, Thr172, Thr183
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Reactivity
- Human, Mouse, Rat, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PRKAA1/PRKAA2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- AMPKα1/2 is a protein encoded by the PRKAA1 gene which is approximately 64 kDa. AMPKα1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, mTOR signalling, AMP-activated protein kinase signalling, the regulation of TP53 activity and longevity regulating pathway. It is a catalytic subunit of AMP-activated protein kinase, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, it activates energy-producing pathways and inhibits energy-consuming processes such as protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPKα1/2 is expressed in nervous system, liver, blood, intestine and kidney. Mutations in the PRKAA1 gene may result in obesity. STJ91581 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of AMPKα1/2 protein.
- Specificity
- AMPKα1/2 Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein.
- Characteristics
- Rabbit polyclonal to AMPKα1/2.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human AMPKalpha1/2 around the non-phosphorylation site of T183/172.
- Isotype
- IgG
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- Application Notes
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WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- PRKAA1/PRKAA2 (AMPK1/AMPK2)
- Alternative Name
- AMPKalpha1/2 (PRKAA1/PRKAA2 Products)
- Synonyms
- AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, PRKAA2 antibody, Prkaa2 antibody
- Molecular Weight
- 63 kDa
- Gene ID
- 5562
- UniProt
- Q13131
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