WNK3 antibody (C-Term)

Catalog No. ABIN6266034

Product Details anti-WNK3 Antibody

Target: WNK3 (WNK Lysine Deficient Protein Kinase 3 (WNK3))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: WNK3 Antibody detects endogenous levels of total WNK3.

Predicted Reactivity: Pig,Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human WNK3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for WNK3

Target: WNK3 (WNK Lysine Deficient Protein Kinase 3 (WNK3))

Alternative Name: WNK3 (WNK3 Products)

Synonyms: fc63h12 antibody, PRKWNK3 antibody, Prkwnk3 antibody, Wnk3-ps antibody, RGD1563131 antibody, wu:fc63h12 antibody, WNK lysine deficient protein kinase 3 antibody, wu:fc63h12 antibody, LOC100363278 antibody, WNK3 antibody, Wnk3 antibody

Background:

Description: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:16275913, PubMed:16275911, PubMed:16357011). Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca2+ influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed:18768590). Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.

Gene: WNK3

Molecular Weight: 198kDa

Gene ID: 65267

UniProt: Q9BYP7