Cadherin 5 antibody (C-Term)

Catalog No. ABIN6265962

Product Details anti-Cadherin 5 Antibody

Target: Cadherin 5 (CDH5)

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: VE-Cadherin Antibody detects endogenous levels of total VE-Cadherin.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human VE-Cadherin, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Cadherin 5 antibody (ABIN6265962)

Meng, Chen, Lei, Han, Zhong, Wang, Liu, Gao, Zhang, Tan, Liu, Zhou, Sun, Yang: "Hsp90β promotes aggressive vasculogenic mimicry via epithelial-mesenchymal transition in hepatocellular carcinoma." in: Oncogene, (2018) (PubMed).

Lv, Phuong, Jin, Li, Xu: "Protection by simvastatin on hyperglycemia-induced endothelial dysfunction through inhibiting NLRP3 inflammasomes." in: Oncotarget, Vol. 8, Issue 53, pp. 91291-91305, (2017) (PubMed).

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: CDH5 (CDH5 Products)

Synonyms: 7B4 antibody, AA408225 antibody, Cd144 antibody, VE-Cad antibody, VECD antibody, VEcad antibody, Vec antibody, wu:fc88a07 antibody, CD144 antibody, cadherin-5 antibody, cadherin 5 antibody, CDH5 antibody, Cdh5 antibody, cdh5 antibody

Background:

Description: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.

Gene: CDH5

Molecular Weight: 120kDa

Gene ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2