USP9X antibody (N-Term)

Catalog No. ABIN6265937

Product Details anti-USP9X Antibody

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP9X antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: USP9X Antibody detects endogenous levels of total USP9X.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human USP9X, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for USP9X

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Alternative Name: USP9X (USP9X Products)

Synonyms: DFFRX antibody, FAF antibody, FAM antibody, RGD1560056 antibody, faf antibody, fam antibody, dffrx antibody, 5730589N07Rik antibody, AA407302 antibody, AA407699 antibody, AL022658 antibody, AL022749 antibody, Dffrx antibody, FAF-X antibody, Fafl antibody, ubiquitin specific peptidase 9, X-linked antibody, ubiquitin specific peptidase 9, X chromosome antibody, USP9X antibody, Usp9x antibody, usp9x antibody

Background:

Description: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111). Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration (PubMed:16322459, PubMed:18254724, PubMed:19135894, PubMed:24607389).

Gene: USP9X

Molecular Weight: 260-290 kDa

Gene ID: 8239

UniProt: Q93008