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UBE2I antibody (N-Term)

UBE2I Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6265848
  • Target See all UBE2I Antibodies
    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
    Binding Specificity
    • 6
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 74
    • 33
    • 32
    • 8
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 63
    • 10
    • 2
    Rabbit
    Clonality
    • 52
    • 23
    Polyclonal
    Conjugate
    • 37
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    This UBE2I antibody is un-conjugated
    Application
    • 51
    • 29
    • 21
    • 9
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    UBE2I Antibody detects endogenous levels of total UBE2I.
    Predicted Reactivity
    Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human UBE2I, corresponding to a region within N-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product UBE2I Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
    Alternative Name
    UBE2I (UBE2I Products)
    Background

    Description: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.

    Gene: UBE2I

    Molecular Weight
    18kDa
    Gene ID
    7329
    UniProt
    P63279
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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