TARBP2 antibody (Internal Region)

Catalog No. ABIN6265719

Product Details anti-TARBP2 Antibody

Target: TARBP2 (TAR (HIV-1) RNA Binding Protein 2 (TARBP2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: TRBP Antibody detects endogenous levels of total TRBP.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRBP, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TARBP2

Target: TARBP2 (TAR (HIV-1) RNA Binding Protein 2 (TARBP2))

Alternative Name: TARBP2 (TARBP2 Products)

Synonyms: trbp antibody, trbp1 antibody, trbp2 antibody, MGC97783 antibody, TARBP2 antibody, LOQS antibody, TRBP antibody, TRBP1 antibody, TRBP2 antibody, fj51e12 antibody, wu:fj51e12 antibody, zgc:63778 antibody, Prbp antibody, TAR (HIV-1) RNA binding protein 2 antibody, TARBP2, RISC loading complex RNA binding subunit antibody, TAR (HIV-1) RNA binding protein 2 L homeolog antibody, TAR (HIV) RNA binding protein 2 antibody, tarbp2 antibody, TARBP2 antibody, tarbp2.L antibody, Tarbp2 antibody

Background:

Description: Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

Gene: TARBP2

Molecular Weight: 43-46 kDa

Gene ID: 6895

UniProt: Q15633

Pathways: Regulatory RNA Pathways, Ribonucleoprotein Complex Subunit Organization