TOP1MT antibody (Internal Region)
-
- Target See all TOP1MT Antibodies
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
-
Binding Specificity
- Internal Region
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This TOP1MT antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Specificity
- TOP1MT Antibody detects endogenous levels of total TOP1MT.
- Predicted Reactivity
- Pig,Zebrafish,Horse,Sheep,Chicken
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human TOP1MT, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product TOP1MT Primary Antibody
-
-
- Application Notes
- WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
-
- Target
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- Alternative Name
- TOP1MT (TOP1MT Products)
- Synonyms
- 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
- Background
-
Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Gene: TOP1MT
- Molecular Weight
- 70 kDa
- Gene ID
- 116447
- UniProt
- Q969P6
-