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TOP1MT antibody (Internal Region)

TOP1MT Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6265672
  • Target See all TOP1MT Antibodies
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Binding Specificity
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 28
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 18
    • 12
    Rabbit
    Clonality
    • 16
    • 14
    Polyclonal
    Conjugate
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TOP1MT antibody is un-conjugated
    Application
    • 11
    • 10
    • 10
    • 9
    • 7
    • 3
    • 3
    • 2
    Western Blotting (WB), ELISA
    Specificity
    TOP1MT Antibody detects endogenous levels of total TOP1MT.
    Predicted Reactivity
    Pig,Zebrafish,Horse,Sheep,Chicken
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human TOP1MT, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product TOP1MT Primary Antibody
  • Application Notes
    WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Alternative Name
    TOP1MT (TOP1MT Products)
    Synonyms
    2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
    Background

    Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

    Gene: TOP1MT

    Molecular Weight
    70 kDa
    Gene ID
    116447
    UniProt
    Q969P6
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