SMCR7L antibody (Internal Region)

Catalog No. ABIN6265187

Product Details anti-SMCR7L Antibody

Target: SMCR7L (Smith-Magenis Syndrome Chromosome Region, Candidate 7-Like (SMCR7L))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMCR7L antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: SMCR7L/MID51 Antibody detects endogenous levels of total SMCR7L/MID51.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SMCR7L/MID51, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SMCR7L

Target: SMCR7L (Smith-Magenis Syndrome Chromosome Region, Candidate 7-Like (SMCR7L))

Alternative Name: MIEF1 (SMCR7L Products)

Synonyms: MGC115280 antibody, HSU79252 antibody, MID51 antibody, MIEF1 antibody, dJ1104E15.3 antibody, A230016E22 antibody, AI452372 antibody, im:7156228 antibody, smcr7l antibody, zgc:162279 antibody, mitochondrial elongation factor 1 antibody, mitochondrial elongation factor 1 S homeolog antibody, MIEF1 antibody, mief1.S antibody, Mief1 antibody, mief1 antibody

Background:

Description: Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.

Gene: MIEF1

Molecular Weight: 48-51 kDa

Gene ID: 54471

UniProt: Q9NQG6