SART3 antibody (C-Term)

Catalog No. ABIN6264927

Product Details anti-SART3 Antibody

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SART3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: SART3 Antibody detects endogenous levels of total SART3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SART3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SART3

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3 (SART3 Products)

Synonyms: SART3 antibody, DKFZp469J2418 antibody, si:ch211-191d15.4 antibody, wu:fc51h03 antibody, DSAP1 antibody, P100 antibody, RP11-13G14 antibody, TIP110 antibody, p110 antibody, p110(nrb) antibody, AU045857 antibody, mKIAA0156 antibody, squamous cell carcinoma antigen recognized by T-cells 3 antibody, squamous cell carcinoma antigen recognized by T-cells 3 L homeolog antibody, squamous cell carcinoma antigen recognized by T cells 3 antibody, SART3 antibody, sart3 antibody, sart3.L antibody, Sart3 antibody

Background:

Description: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity).

Gene: SART3

Molecular Weight: 110kDa

Gene ID: 9733

UniProt: Q15020

Pathways: Ribonucleoprotein Complex Subunit Organization