RPS27A antibody (Internal Region)

Catalog No. ABIN6264854

Product Details anti-RPS27A Antibody

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS27A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: RPS27A Antibody detects endogenous levels of total RPS27A.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RPS27A, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RPS27A

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Alternative Name: RPS27A (RPS27A Products)

Synonyms: CEP80 antibody, S27A antibody, UBA80 antibody, UBC antibody, UBCEP1 antibody, UBCEP80 antibody, 0610006J14Rik antibody, Uba52 antibody, Ubb antibody, Ubc antibody, CG5271 antibody, DUb80 antibody, Dmel\\CG5271 antibody, Dub80 antibody, UB3-D antibody, Ubi-f80 antibody, Ubi-m antibody, Ubiq antibody, l(2)04820 antibody, l(2)31Ei antibody, mfs(2)31 antibody, mfs(2)48 antibody, mfs48 antibody, Ubiz antibody, hm:zeh0386 antibody, ik:tdsubc_1f2 antibody, tdsubc_1f2 antibody, xx:tdsubc_1f2 antibody, zgc:66168 antibody, rps27a antibody, rps27a.L antibody, ribosomal protein S27a antibody, ribosomal protein S27A antibody, Ribosomal protein S27A antibody, ubiquitin-40S ribosomal protein S27a antibody, ribosomal protein S27a S homeolog antibody, RPS27A antibody, Rps27a antibody, RpS27A antibody, rps27a antibody, LOC105381543 antibody, rps27a.S antibody

Background:

Description: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Gene: RPS27A

Molecular Weight: 9kDa

Gene ID: 6233

UniProt: P62979

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation