PRDM14 antibody (N-Term)

Catalog No. ABIN6264408

Product Details anti-PRDM14 Antibody

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Specificity: PRDM14 Antibody detects endogenous levels of total PRDM14.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PRDM14, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:20-1:100, CHIP 1:20-1:100, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PRDM14

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Alternative Name: PRDM14 (PRDM14 Products)

Synonyms: PFM11 antibody, PRDM14 antibody, prmd14 antibody, fc16b12 antibody, wu:fc16b12 antibody, PR/SET domain 14 antibody, PR domain containing 14 antibody, PRDM14 antibody, Prdm14 antibody, prdm14 antibody

Background:

Description: Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Gene: PRDM14

Molecular Weight: 64kDa

Gene ID: 63978

UniProt: Q9GZV8