POLI antibody (N-Term)
-
- Target See all POLI Antibodies
- POLI (Polymerase (DNA Directed) iota (POLI))
-
Binding Specificity
- N-Term
-
Reactivity
- Human, Rat, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This POLI antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- POLI Antibody detects endogenous levels of total POLI.
- Predicted Reactivity
- Rabbit
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human POLI, corresponding to a region within N-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product POLI Primary Antibody
-
anti-Polymerase (DNA Directed) iota (POLI) (AA 616-715) antibody
POLI Reactivity: Human WB, ELISA Host: Mouse Monoclonal 8G9 unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 458-711) antibodyPOLI Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibodyPOLI Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 458-740) antibodyPOLI Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1B9 unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 50-350) antibodyPOLI Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (C-Term) antibodyPOLI Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 51-150) antibodyPOLI Reactivity: Rat ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 143-192) antibodyPOLI Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Monkey, Pig, Bat WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibodyPOLI Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Rabbit, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (DNA Directed) iota (POLI) (AA 673-701), (C-Term) antibodyPOLI Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
-
- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
-
- Target
- POLI (Polymerase (DNA Directed) iota (POLI))
- Alternative Name
- POLI (POLI Products)
- Background
-
Description: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Gene: POLI
- Molecular Weight
- 80kDa
- Gene ID
- 11201
- UniProt
- Q9UNA4
- Pathways
- DNA Damage Repair
-
