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POLH antibody (Internal Region)

POLH Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6264337
  • Target See all POLH Antibodies
    POLH (Polymerase (DNA Directed), eta (POLH))
    Binding Specificity
    • 15
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Internal Region
    Reactivity
    • 42
    • 11
    • 11
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 42
    • 3
    Rabbit
    Clonality
    • 44
    • 1
    Polyclonal
    Conjugate
    • 18
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLH antibody is un-conjugated
    Application
    • 32
    • 13
    • 13
    • 11
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Specificity
    POLH Antibody detects endogenous levels of total POLH.
    Predicted Reactivity
    Sheep
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human POLH, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product POLH Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    POLH (Polymerase (DNA Directed), eta (POLH))
    Alternative Name
    POLH (POLH Products)
    Synonyms
    POLH antibody, fc50a12 antibody, wu:fc50a12 antibody, zgc:136881 antibody, zgc:152642 antibody, RAD30 antibody, RAD30A antibody, XP-V antibody, XPV antibody, polymerase (DNA directed), eta S homeolog antibody, DNA polymerase eta antibody, polymerase (DNA directed), eta antibody, putative DNA polymerase eta antibody, polymerase (DNA directed), eta (RAD 30 related) antibody, polh.S antibody, POLH antibody, polh antibody, Tb10.70.6190 antibody, LINJ_21_0690 antibody, Polh antibody, LOC100443019 antibody
    Background

    Description: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).

    Gene: POLH

    Molecular Weight
    78kDa
    Gene ID
    5429
    UniProt
    Q9Y253
    Pathways
    DNA Damage Repair
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