PIAS3 antibody (N-Term)

Catalog No. ABIN6264198

Product Details anti-PIAS3 Antibody

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PIAS3 Antibody detects endogenous levels of total PIAS3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PIAS3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: PIAS3 (PIAS3 Products)

Synonyms: PIAS3 antibody, Pias3l antibody, ZMIZ5 antibody, protein inhibitor of activated STAT 3 antibody, protein inhibitor of activated STAT, 3 antibody, protein inhibitor of activated STAT 3 L homeolog antibody, PIAS3 antibody, pias3 antibody, Pias3 antibody, pias3.L antibody

Background:

Description: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).

Gene: PIAS3

Molecular Weight: 68kDa

Gene ID: 10401

UniProt: Q9Y6X2

Pathways: JAK-STAT Signaling