PERK antibody (C-Term)

Catalog No. ABIN6264143

Product Details anti-PERK Antibody

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PERK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PERK Antibody detects endogenous levels of total PERK.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PERK, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PERK antibody (ABIN6264143)

Lu, Chen, Tang, Wang, Zhang: "GRP78 silencing enhances hyperoxia-induced alveolar epithelial cell apoptosis via CHOP pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1493-1501, (2018) (PubMed).

Liu, Shen, Wang, Liu, Zheng, Luo: "EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes." in: Journal of cellular biochemistry, (2018) (PubMed).

Yang, He, Li, Zhang, He, Duan, Huang, Xiang: "SWNHs (Single-Wall Carbon Nanohorns) Supervises Endoplasmic Reticulum (ER) Stress in Hepatocellular Carcinoma." in: Journal of nanoscience and nanotechnology, Vol. 18, Issue 10, pp. 6740-6745, (2018) (PubMed).

Target Details for PERK

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Alternative Name: EIF2AK3 (EIF2AK3 Products)

Synonyms: EIF2AK3 antibody, AI427929 antibody, Pek antibody, Perk antibody, PEK antibody, PERK antibody, WRS antibody, eukaryotic translation initiation factor 2 alpha kinase 3 antibody, EIF2AK3 antibody, Eif2ak3 antibody

Background:

Description: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Gene: EIF2AK3

Molecular Weight: 125 kDa

Gene ID: 9451

UniProt: Q9NZJ5

Pathways: Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response