NSUN2 antibody (Internal Region)

Catalog No. ABIN6263746

Product Details anti-NSUN2 Antibody

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: NSUN2 Antibody detects endogenous levels of total NSUN2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NSUN2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Alternative Name: NSUN2 (NSUN2 Products)

Synonyms: D13Wsu123e antibody, Misu antibody, MISU antibody, MRT5 antibody, SAKI antibody, TRM4 antibody, RGD1311954 antibody, NOL1/NOP2/Sun domain family member 2 antibody, NOP2/Sun RNA methyltransferase family member 2 antibody, NOP2/Sun RNA methyltransferase family, member 2 antibody, NOP2/Sun RNA methyltransferase family member 2 S homeolog antibody, Nsun2 antibody, NSUN2 antibody, nsun2.S antibody

Background:

Description: RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

Gene: NSUN2

Molecular Weight: 90-100 kDa

Gene ID: 54888

UniProt: Q08J23