PPP1R12A antibody (C-Term)

Catalog No. ABIN6263491

Product Details anti-PPP1R12A Antibody

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: MYPT1 Antibody detects endogenous levels of total MYPT1.

Predicted Reactivity: Zebrafish,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MYPT1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PPP1R12A antibody (ABIN6263491)

Xiaojun, Yan, Hong, Xianghong, Shifeng, Dingjie, Xuemin, Lijuan, Bonan, Zhongqiu, Ruimin, Brann, Fang: "Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica." in: Scientific reports, Vol. 6, pp. 32257, (2018) (PubMed).

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A (PPP1R12A Products)

Synonyms: 1200015F06Rik antibody, 5730577I22Rik antibody, AA792106 antibody, AV099298 antibody, D10Ertd625e antibody, Mypt1 antibody, M130 antibody, MBS antibody, MYPT1 antibody, M110 antibody, MBSP antibody, protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody, protein phosphatase 1 regulatory subunit 12A antibody, protein phosphatase 1, regulatory subunit 12A antibody, Ppp1r12a antibody, PPP1R12A antibody

Background:

Description: Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Gene: PPP1R12A

Molecular Weight: 115 kDa

Gene ID: 4659

UniProt: O14974

Pathways: M Phase