MLKL antibody (Internal Region)

Catalog No. ABIN6263279

Product Details anti-MLKL Antibody

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: MLKL Antibody detects endogenous levels of total MLKL.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MLKL, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-MLKL antibody (ABIN6263279)

Fang, Cao, Wang, Ye, Shen, Li, Hu, Gao: "Alterations in necroptosis during ALDH2‑mediated protection against high glucose‑induced H9c2 cardiac cell injury." in: Molecular medicine reports, Vol. 18, Issue 3, pp. 2807-2815, (2018) (PubMed).

Target Details for MLKL

Target: MLKL (Mixed Lineage Kinase Domain-Like (MLKL))

Alternative Name: MLKL (MLKL Products)

Synonyms: MLKL antibody, 9130019I15Rik antibody, mixed lineage kinase domain like pseudokinase antibody, mixed lineage kinase domain-like antibody, MLKL antibody, mlkl antibody, Mlkl antibody

Background:

Description: Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Gene: MLKL

Molecular Weight: 54kDa

Gene ID: 197259

UniProt: Q8NB16