MED14 antibody (Internal Region)

Catalog No. ABIN6263189

Product Details anti-MED14 Antibody

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: MED14 Antibody detects endogenous levels of total MED14.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MED14, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MED14

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Alternative Name: MED14 (MED14 Products)

Synonyms: CG12031 antibody, Dmel\\CG12031 antibody, Med14 antibody, Trap170 antibody, dTRAP170 antibody, p160 antibody, EXLM1 antibody, crsp150 antibody, crsp2 antibody, trap170 antibody, wu:fd21c05 antibody, wu:fk28h11 antibody, CRSP2 antibody, NV18605 antibody, CRSP150 antibody, CSRP antibody, CXorf4 antibody, DRIP150 antibody, RGR1 antibody, TRAP170 antibody, 9930001L01Rik antibody, AU041628 antibody, Crsp2 antibody, ENSMUSG00000073278 antibody, Gm641 antibody, ORF1 antibody, RGD1560170 antibody, Mediator complex subunit 14 antibody, mediator complex subunit 14 antibody, mediator of RNA polymerase II transcription subunit 14 antibody, transcriptional regulation antibody, MED14 antibody, med14 antibody, LOC100113592 antibody, Med14 antibody, RGR1 antibody

Background:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene: MED14

Molecular Weight: 160kDa

Gene ID: 9282

UniProt: O60244

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha