LAMP2 antibody (C-Term)

Catalog No. ABIN6262907

Product Details anti-LAMP2 Antibody

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: LAMP2 Antibody detects endogenous levels of total LAMP2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LAMP2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: LAMP2 (LAMP2 Products)

Synonyms: CD107b antibody, LAMP-2 antibody, LAMPB antibody, LGP110 antibody, Lamp-2 antibody, Lamp-2a antibody, Lamp-2b antibody, Lamp-2c antibody, Mac3 antibody, cd107b antibody, lamp-2 antibody, lampb antibody, LAMP2 antibody, lamp2 antibody, DKFZp459J126 antibody, LGP-B antibody, sb:cb587 antibody, wu:fb14a06 antibody, zgc:103532 antibody, lysosomal associated membrane protein 2 antibody, lysosomal-associated membrane protein 2 antibody, lysosomal-associated membrane protein 2 S homeolog antibody, lysosomal membrane glycoprotein 2 antibody, LAMP2 antibody, Lamp2 antibody, lamp2.S antibody, lamp2 antibody

Background:

Description: Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation, antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820). Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).

Gene: LAMP2

Molecular Weight: 45kDa

Gene ID: 3920

UniProt: P13473

Pathways: Autophagy