EIF3L antibody (N-Term)

Catalog No. ABIN6262447

Product Details anti-EIF3L Antibody

Target: EIF3L (Eukaryotic Translation Initiation Factor 3, Subunit L (EIF3L))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: IF3EI Antibody detects endogenous levels of total IF3EI.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IF3EI, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF3L

Target: EIF3L (Eukaryotic Translation Initiation Factor 3, Subunit L (EIF3L))

Alternative Name: EIF3L (EIF3L Products)

Synonyms: wu:fb66c05 antibody, zgc:64147 antibody, EIF3EIP antibody, EIF3S11 antibody, EIF3S6IP antibody, HSPC021 antibody, HSPC025 antibody, MSTP005 antibody, 0610011H21Rik antibody, D15N1e antibody, Eif3eip antibody, Eif3ip antibody, Eif3s6ip antibody, HSP-66Y antibody, PAF67 antibody, eif3eip antibody, eif3s6ip antibody, eukaryotic translation initiation factor 3, subunit 6 interacting protein antibody, eukaryotic translation initiation factor 3 subunit L antibody, eukaryotic translation initiation factor 3, subunit L antibody, eukaryotic translation initiation factor 3 subunit L L homeolog antibody, eif3s6ip antibody, EIF3L antibody, Eif3l antibody, eif3l.L antibody

Background:

Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3L

Molecular Weight: 67kDa

Gene ID: 51386

UniProt: Q9Y262

Pathways: Ribonucleoprotein Complex Subunit Organization