H1FOO antibody (Internal Region)

Catalog No. ABIN6262247

Product Details anti-H1FOO Antibody

Target: H1FOO (H1 Histone Family, Member O, Oocyte-Specific (H1FOO))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H1FOO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Histone H1 Antibody detects endogenous levels of total Histone H1.

Predicted Reactivity: Bovine,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Histone H1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for H1FOO

Target: H1FOO (H1 Histone Family, Member O, Oocyte-Specific (H1FOO))

Alternative Name: H1FOO (H1FOO Products)

Synonyms: osH1 antibody, C86609 antibody, H1fo antibody, H1oo antibody, b4 antibody, RGD1566236 antibody, H1 histone family member O oocyte specific antibody, H1 histone family, member O, oocyte-specific antibody, H1 histone family member O oocyte specific S homeolog antibody, H1FOO antibody, H1foo antibody, h1foo.S antibody

Background:

Description: May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling (By similarity).

Gene: H1FOO

Molecular Weight: 21kDa

Gene ID: 132243

UniProt: Q8IZA3