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GSDMD antibody

GSDMD Reactivity: Human WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6262106
  • Target See all GSDMD Antibodies
    GSDMD (Gasdermin D (GSDMD))
    Reactivity
    • 19
    • 2
    • 1
    Human
    Host
    • 16
    • 4
    Rabbit
    Clonality
    • 16
    • 4
    Polyclonal
    Conjugate
    • 16
    • 2
    • 1
    • 1
    This GSDMD antibody is un-conjugated
    Application
    • 15
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    GSDMD Antibody detects endogenous levels of total GSDMD.
    Predicted Reactivity
    Pig,Bovine,Horse,Dog
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human GSDMD.
    Isotype
    IgG
    Top Product
    Discover our top product GSDMD Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200 IF/ICC 1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    GSDMD (Gasdermin D (GSDMD))
    Alternative Name
    GSDMD (GSDMD Products)
    Synonyms
    GSDMDC1 antibody, GSDMD antibody, DF5L antibody, DFNA5L antibody, 1810036L03Rik antibody, AW558049 antibody, Dfna5l antibody, Gsdmdc1 antibody, M2-4 antibody, gasdermin D antibody, GSDMD antibody, Gsdmd antibody
    Background

    Description: Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).

    Gene: GSDMD

    Molecular Weight
    50-53 kDa
    Gene ID
    79792
    UniProt
    P57764
    Pathways
    Inflammasome
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