GCLC antibody (N-Term)

Catalog No. ABIN6261919

Product Details anti-GCLC Antibody

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: GCLC Antibody detects endogenous levels of total GCLC.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GCLC, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-GCLC antibody (ABIN6261919)

Zou, Wang, Peng, Wei: "Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5987183, (2017) (PubMed).

Li, Sun, Liu, Zhao, Shao: "Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 102, pp. 93-101, (2017) (PubMed).

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC (GCLC Products)

Synonyms: GCLC antibody, gclc antibody, MGC146287 antibody, 2259 antibody, CG2259 antibody, DmGCLC antibody, DmGCS antibody, DmGCSh antibody, Dmel\\CG2259 antibody, GCL antibody, GCLc antibody, GCSh antibody, Gcs antibody, Gcsh antibody, DDBDRAFT_0186120 antibody, DDBDRAFT_0231403 antibody, DDB_0186120 antibody, DDB_0231403 antibody, GCS antibody, GLCL antibody, GLCLC antibody, D9Wsu168e antibody, GLCL-H antibody, Ggcs-hs antibody, Glclc antibody, cb1049 antibody, fe36e11 antibody, wu:fe36e11 antibody, glutamate-cysteine ligase catalytic subunit antibody, glutamate-cysteine ligase, catalytic subunit antibody, Glutamate-cysteine ligase catalytic subunit antibody, gamma glutamylcysteine synthetase antibody, glutamate-cysteine ligase, catalytic subunit L homeolog antibody, glutamate-cysteine ligase, catalytic subunit S homeolog antibody, gamma-glutamylcysteine synthetase antibody, GCLC antibody, gclc antibody, Gclc antibody, LbGCS antibody, gcsA antibody, gclc.L antibody, gclc.S antibody, GSH1 antibody

Background:

Description: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Gene: GCLC

Molecular Weight: 72 kDa

Gene ID: 2729

UniProt: P48506

Pathways: Cell RedoxHomeostasis