Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PHD1 antibody (N-Term)

EGLN2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6261465
  • Target See all PHD1 (EGLN2) Antibodies
    PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
    Binding Specificity
    • 15
    • 10
    • 8
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 57
    • 32
    • 18
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 68
    • 3
    • 1
    Rabbit
    Clonality
    • 68
    • 4
    Polyclonal
    Conjugate
    • 28
    • 7
    • 7
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PHD1 antibody is un-conjugated
    Application
    • 61
    • 34
    • 15
    • 14
    • 13
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    EGLN2 Antibody detects endogenous levels of total EGLN2.
    Predicted Reactivity
    Pig,Rabbit,Dog
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human EGLN2, corresponding to a region within N-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product EGLN2 Primary Antibody
  • Application Notes
    WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
    Alternative Name
    EGLN2 (EGLN2 Products)
    Synonyms
    egln2 antibody, phd2 antibody, PHD1 antibody, 0610011A13Rik antibody, C85656 antibody, Hif-p4h-1 antibody, Ier4 antibody, Phd1 antibody, SM-20 antibody, HIF-PH1 antibody, HPH-1 antibody, HPH-3 antibody, PHD-1 antibody, EIT6 antibody, HIFPH1 antibody, egl-9 family hypoxia-inducible factor 1 antibody, egl-9 family hypoxia inducible factor 2 antibody, Egl nine homolog 2 antibody, egl-9 family hypoxia-inducible factor 2 L homeolog antibody, egl-9 family hypoxia-inducible factor 2 antibody, egln1 antibody, EGLN2 antibody, egln2 antibody, egln2.L antibody, Egln2 antibody
    Background

    Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.

    Gene: EGLN2

    Molecular Weight
    44 kDa
    Gene ID
    112398
    UniProt
    Q96KS0
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis
You are here:
Support