EGLN1 antibody (Internal Region)

Catalog No. ABIN6261464

Product Details anti-EGLN1 Antibody

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PHD2 Antibody detects endogenous levels of total PHD2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PHD2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1 (EGLN1 Products)

Synonyms: EGLN1 antibody, egln2 antibody, phd2 antibody, zgc:92314 antibody, AI503754 antibody, C1orf12 antibody, HIF-PH2 antibody, HPH-2 antibody, Hif-p4h-2 antibody, ORF13 antibody, Phd2 antibody, SM-20 antibody, PHD-2 antibody, PHD2 antibody, ECYT3 antibody, HIFPH2 antibody, HPH2 antibody, SM20 antibody, ZMYND6 antibody, egl nine homolog 1 (C. elegans) antibody, egl-9 family hypoxia inducible factor 1 antibody, egl-9 family hypoxia-inducible factor 1 S homeolog antibody, egl-9 family hypoxia-inducible factor 1b antibody, egl-9 family hypoxia-inducible factor 1 antibody, EGLN1 antibody, egln1.S antibody, egln1b antibody, Egln1 antibody

Background:

Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.

Gene: EGLN1

Molecular Weight: 46kDa

Gene ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect