TPX2 antibody (C-Term)

Catalog No. ABIN6261299

Product Details anti-TPX2 Antibody

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: DIL-2 Antibody detects endogenous levels of total DIL-2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DIL-2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TPX2

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Alternative Name: TPX2 (TPX2 Products)

Synonyms: C20orf1 antibody, C20orf2 antibody, DIL-2 antibody, DIL2 antibody, FLS353 antibody, GD:C20orf1 antibody, HCA519 antibody, HCTP4 antibody, REPP86 antibody, p100 antibody, 2610005B21Rik antibody, TPX2 antibody, dil-2 antibody, dil2 antibody, hctp4 antibody, repp86 antibody, tpx2 antibody, TPX2, microtubule nucleation factor antibody, TPX2, microtubule-associated antibody, TPX2, microtubule-associated, homolog (Xenopus laevis) antibody, TPX2, microtubule-associated S homeolog antibody, TPX2, microtubule-associated L homeolog antibody, TPX2 antibody, Tpx2 antibody, tpx2.S antibody, tpx2.L antibody

Background:

Description: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).

Gene: TPX2

Molecular Weight: 85 kDa

Gene ID: 22974

UniProt: Q9ULW0