CHCHD4 antibody (N-Term)

Catalog No. ABIN6260791

Product Details anti-CHCHD4 Antibody

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHCHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: CHCHD4 Antibody detects endogenous levels of total CHCHD4.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CHCHD4, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CHCHD4

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Alternative Name: CHCHD4 (CHCHD4 Products)

Synonyms: 2410012P20Rik antibody, 2810014D17Rik antibody, AI838740 antibody, MIA40 antibody, TIMM40 antibody, mia40 antibody, timm40 antibody, chchd4 antibody, chchd4a antibody, fc10g04 antibody, wu:fc10g04 antibody, zgc:100849 antibody, si:dkey-202e22.1 antibody, chchd4b antibody, CaO19.2977 antibody, coiled-coil-helix-coiled-coil-helix domain containing 4 antibody, coiled-coil-helix-coiled-coil-helix domain containing 4 L homeolog antibody, coiled-coil-helix-coiled-coil-helix domain containing 4a antibody, coiled-coil-helix-coiled-coil-helix domain containing 4 S homeolog antibody, Mia40p antibody, Chchd4 antibody, CHCHD4 antibody, chchd4 antibody, chchd4.L antibody, chchd4a antibody, chchd4.S antibody, LOC100361898 antibody, MIA40 antibody

Background:

Description: Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364, PubMed:23676665). Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23676665). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).

Gene: CHCHD4

Molecular Weight: 22 kDa

Gene ID: 131474

UniProt: Q8N4Q1