CDC34 antibody (Internal Region)

Catalog No. ABIN6260683

Product Details anti-CDC34 Antibody

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC34 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: CDC34 Antibody detects endogenous levels of total CDC34.

Predicted Reactivity: Pig,Bovine,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CDC34, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CDC34

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Alternative Name: CDC34 (CDC34 Products)

Synonyms: E2-CDC34 antibody, UBC3 antibody, UBCH3 antibody, UBE2R1 antibody, AI327276 antibody, e2-cdc34 antibody, ubc3 antibody, ube2r1 antibody, CDC34 antibody, DNA6 antibody, Ube2r2 antibody, zgc:55452 antibody, fb34h09 antibody, wu:fb34h09 antibody, wu:fe17e09 antibody, zgc:91847 antibody, cell division cycle 34 antibody, cell division cycle 34 L homeolog antibody, SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 antibody, SCF E2 ubiquitin-protein ligase catalytic subunit antibody, cell division cycle 34 homolog (S. cerevisiae) a antibody, cell division cycle 34 homolog (S. cerevisiae) b antibody, CDC34 antibody, Cdc34 antibody, cdc34.L antibody, cdc34 antibody, cdc34a antibody, cdc34b antibody

Background:

Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421).

Gene: CDC34

Molecular Weight: 26 kDa

Gene ID: 997

UniProt: P49427